Hi Henry We have used 5-10uM with success. Larry At 07:43 PM 1/22/2001 -0500, you wrote: >Would someone with experience using CFSE, particularly with murine B >cells, confirm >the correct dose? The one published >study with B cells that I found states that a dose of 10 nM/ml was >used. This seems >to be about 1000 fold less than the >usual dose. > > >Henry H. Wortis, M.D., Professor >Department of Pathology, >Director, Graduate Program in Immunology >Tufts University School of Medicine >136 Harrison Ave. >Boston MA 02111 >Phone 617-636-6718 >FAX 617-636-2990 Larry W. Arnold, Ph.D. Res. Assoc. Prof. Director, Flow Cytometry Facility Department of Microbiology and Immunology Lineberger Comprehensive Cancer Center CB# 7290 University of North Carolina at Chapel Hill Chapel Hill, NC 27599 Phone: 919-966-1530 FAX: 919-962-8103
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