Tony,
On the Purdue Cytometry CD volume five, there is a fluidics
troubleshooting flow chart that I created to help users troubleshoot fluidic
problems on the Facscan. I believe it is in the Tools/Utilities ; useful
tools, section. If you don't have this CD, I can send you a copy, or give me
a call and we can probably fix this over the phone. I have read Ray Hicks
message and if his suggestions didn't work, try disconnecting the black
(waste) piece of tubing from the top of the flow cell. Place a piece of
1/16"ID tubing on the barb fitting then run it a separate waste container.
This will by-pass the installed waste system. If you now have flow then you
have a clog in your waste system and we can help you fix that. If your
sample probe has a persistent clog that won't let go after following Ray's
suggestions, then you might want to try using a piece of .005" wire to
unclog the sample probe. Instructions on how to do this are in the
troubleshooting flow chart. Hope this helps.
Regards,
Raymond Lannigan
Tritech Inc.
2014 Renard Ct. Suite I
Annapolis, MD 21401
1-410-266-1522
1-800-886-7004
www.tritechinc.com
-----Original Message-----
From: Tony Schountz <tschount@mesastate.edu>
To: cyto-inbox
Date: Thursday, January 18, 2001 3:52 PM
Subject: No flow on cytometer
>
>Our BD FACScan seems to have flow problems. It hasn't been used for a
>couple of months, so I think it's just clogged lines. But since we don't
>have a service contract I was hoping the group might have some
>suggestions as to get the the lines unclogged. In the event this isn't
>the problem, what else should I check out?
>
>Thanks,
>
>Tony
>
>--
>Tony Schountz, Ph.D.
>Department of Biological Sciences
>Mesa State College
>mailto:tschount@mesastate.edu
>
>
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