Hi all, Some of my colleagues in the lab are trying to set up a protocol for studying SP (side population) cells on a FACSVantage, using Hoechst labelling. They have been trying for a few weeks now (using mouse bone marrow cells, but ultimately they want to use human cells), but have problems with setting the UV right, and getting the typical SP pattern. Of course they do not know whether the problem lies with their labelling or with the cytometer settings. Are there people who have experience with this labelling, and can help my colleagues with references and practical guidance. Are there labs close to Belgium where they can come and learn at the spot how to set the Vantage? Thanks a lot! Tessa Kerre Ghent University Hospital - Immunology Department Belgium
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