APC-conjugated antibodies

From: Sathi (sathi@repromedix.com)
Date: Fri Jan 12 2001 - 15:01:11 EST


I like to do a 4 color-analysis on Coulter-Epics/XL for lymphocytes.   For
one of the marker, I have an APC-conjugates antibody.  Could any one tell
me the emission/excitations spectrum for APC-fluorochrome and which channel
should I use for APC on Coulter Epics/XL cytometer.   I will be using FITC
and PE for other channels and the info would help me to choose the fourth
color (ECD or PC-5).

I would appreciate any helpful comments.

Thanks
Sathi
-------------------------------------------------------------
T. Sathiyaseelan, B.V.Sc., Ph.D.
Repromedix Corp.
sathi@repromedix.com



This archive was generated by hypermail 2b29 : Sun Jan 05 2003 - 19:01:02 EST