Thomas, First do the RBC lysis, then label your cells with your monoclonal cocktail (use an azide-containing buffer to "freeze" the surface markers), and AFTERWARDS fix in formaldehyde (we routinely use 1% PFA in PBS and keep the samples in this buffer in the fridge until FACS analysis). Please feel free to ask if you need more details. Karim Miller.Thomas@EPAMAIL.EPA.GOV wrote: > I was wondering if anyone knows how long I can store heparinized mouse > blood before the CD4 & CD8 counts will be "off". I'm running an experiment > with about 80 mice and will not be able to run the CD4-CD8 counts on the > FACS for several days. Can I use a dilute solution of formaldehyde to fix > the sample after I lyse the RBCs or will that destroy the epitope for the > antibody? I'm open to any ideas.
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