Heparinized mouse blood storage? Formaldehyde treated?

From: Miller.Thomas@EPAMAIL.EPA.GOV
Date: Tue Jan 09 2001 - 14:10:34 EST


I was wondering if anyone knows how long I can store heparinized mouse
blood before the CD4 & CD8 counts will be "off".  I'm running an experiment
with about 80 mice and will not be able to run the CD4-CD8 counts on the
FACS for several days. Can I use a dilute solution of formaldehyde to fix
the sample after I lyse the RBCs or will that destroy the epitope for the
antibody?  I'm open to any ideas.



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