Hi, We have been working on an opsonophagocytosis assay to detect anti-Pneumococcal antibodies in serum using DMF differentiated HL-60 cells as the effector cells. We really have read many papers on this and related subjects. However, we have only found passing references to the problems which we are encountering. We have also found some confusion in published methods where the same authors use very different methods over a number of papers. We are finding phagocytosis in the presence of complement only (baby rabbit serum). Different batches have been tried. How long can you passage HL-60 cells and expect them (after differentiation) to still be useable in such an assay? Is anyone running assays like this and could offer some advice on methods? After a particularly frustrating few weeks, we would be grateful for any help on this matter. Jenny Jenny Bryant Flow Cytometry Australian Red Cross Blood Service - NSW/ACT 153 Clarence St Sydney, NSW 2000, AUSTRALIA .._|\ Ph: 61 2 9229 4341 / \ FAX: 61 2 9229 4521 \_.-._/<<<<<< E-mail: jbryant@arcbs.redcross.org.au v
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