>From experience (20 years!) the cardiac puncture is a one off!! The mouse is euthanised after the procedure, i.e. it is not allowed to wakeup! Lesley Mrs. Lesley Barber Edith Margaret Collie Centre for Blood Cell Therapies Div. Haematology/Medical Oncology Peter MacCallum Cancer Institute East Melbourne, 3002 VICTORIA , AUSTRALIA Tel 61 3 9656 1957 or: Tel 61 3 9656 1111 page 1271 Fax 61 3 9656 1811 > -----Original Message----- > From: rafael nunez [SMTP:rafaeln@vetvir.unizh.ch] > Sent: Friday, December 08, 2000 7:17 PM > To: Cytometry Mailing List > Subject: Re: Venous versus cardiac blood collection in mice > > > Dear flow-ers, I am very much surprise and in a shock after learning from > the board that some people can take up to 1 ml from the heart of a mice. > Just from the mouse point of view, 1 ml represent about 20-25% of the > whole > blood volumen and even for an expert mice heart surgeon, to take such > volumen will produce a shock in the animal. Just for comparison, if you > take from a human donor, 25% of blood, it means that you are producing a > non-reversible hypovolemic shock in a patient. I imagine that the lawsuits > will flood your place. I do not know the rules for animal experimentation > in other places but the rules that I do know, only allow to take not more > than 200 ul of blood each three weeks. I imagine that you will have to > write to the board that review your application for animal > experimentation, > your procedures and how you will treat your animals. Taking 1 ml of blood > from the heart is not VIP treatment. I really question the legality, > benefits and scientific grounds for such invasive procedures. > Rafael > > > \|/ > (o o) > ________________________________oOo__(_)__oOo_____________________________ > ____ > ___/\_ | Rafael Nunez > mailto:rafaeln@vetvir.unizh.ch > / o \/| | University Inst.for Virology http://www.vetvir.unizh.ch/ > / _| | Winterthurerstr. 266a Telephone: (+41) 1 6358710 > /_/\__/-\/ | 8057 Zurich SWITZERLAND Faximile : (+41) 1 6358911 > __________________________________________________________________________ > ____ > > > > > >
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