David, If I understand you well, in order to be able to use the KJ1-26, we would have to cross TCRa and TCRb mice first? I.e. if we'd just ordered TCRb mice for example, we would not see any staining with the KJ1-26? Thanks again Karim -------- Original Message -------- Subject: RE: DO11.10 and KJ1-26 Date: Thu, 7 Dec 2000 11:58:46 -0500 From: "Topham, David" <David_Topham@URMC.Rochester.edu> To: cyto-inbox I would check both alpha and beta expression. We just had an incident with Jackson and the OT-I CD8 Tg mice. The OT-I mice Jackson had been sending out do not express the Tg alpha chain, only the beta chain. Jackson types the mice using Vbeta expression only, so they did not notice the mistake. The KJ26 should see only the combination of the Tg alpha and beta though. David J. Topham, Ph.D. Center for Vaccine Biology & Immunology Aab Institute for Biomedical Sciences University of Rochester Medical Center 601 Elmwood Avenue, Box 609 Rochester, NY 14642-8609 Tel. 716 273-1403 FAX 716 273-2452 E-mail: david_topham@urmc.rochester.edu > ---------- > From: Karim Vermaelen > Reply To: Karim.Vermaelen@rug.ac.be > Sent: Tuesday, December 5, 2000 11:45 AM > To: Cytometry Mailing List > Subject: DO11.10 and KJ1-26 > > > Hi there, > > This is a borderline flow question... We'd like to get some OVA-TCR > transgenic mice (BALB/c b.g) from Jackson. How should we base our choice > when deciding for TCRa OR TCRb strains? Does the anti-clonotypic KJ1-26 > MAb discriminate between those 2 strains? > Thanks for any advice!! > > Karim > > Respiratory Diseases > Ghent University Hospital > Belgium > >
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