Hi Chris, unless there's a good reason for it, I would suggest washing just once; the more you manhandle cells the more you compromise the viability. Instead of resuspending the cells in PBS/BSA you could try medium ( will however give you increased autofl. which will not be a problem if you have a good signal above that for the stained population).Also sort into medium. The settings for the Vantage are fine,(I've used a 70um nozzle with no adverse effects) unless of course you have a bad nozzle. regards Ann At 09:49 05.12.00 -0800, you wrote: > >We are having problems with the viability of our Embryonic Stem Cells >after sorting. I am using a VantageSE at 9psi, 21K ddf, and a 100u >nozzle. Prior to sorting, these cells have been stained, spun, and >washed 5 times. Is anyone successfully sorting this type of cell?? > >chris > >trussell@gnf.org > >
This archive was generated by hypermail 2b29 : Sat Mar 10 2001 - 19:31:40 EST