Embyonic Stem Cells

From: trussell (trussell@gnf.org)
Date: Tue Dec 05 2000 - 12:49:09 EST

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We are having problems with the viability of our Embryonic Stem Cells
after sorting.  I am using a VantageSE at 9psi, 21K ddf, and a 100u
nozzle.  Prior to sorting, these cells have been stained, spun, and
washed 5 times.  Is anyone successfully sorting this type of cell??

chris

trussell@gnf.org

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