Cytokine Immunoassays on FACS Vantage

From: MSchwa3722@aol.com
Date: Mon Nov 20 2000 - 19:15:27 EST


I was interested in knowing if anyone has used any quantitative bead-based
assays on the FACS Vantage.  I'm looking at Multiflow and ImmunoFlow from
BioE for the analysis of various cytokines from cell supernatents.  Each kit
comes with only enough standard control material for 5 runs.  Their test kits
can do 100 tests.  I feel the changes in daily alignment on the Vantage would
mandate running standards each time you ran the assay.  As opposed to the
FACS Scan, with a fixed alignment, where you might be able to use the same
standard curve repeatedly for the same lot.

I'd appreciate any feedback.
Michael Schwartz
Children's Hospital of Orange County
Orange, CA



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