We stain mitochondria (we've used mitotracker red) and then isolate them by a simple cell lysis procedure which keeps them viable but removes them from the cytoplasm. Then, we sort to isolate pure mitochondria (to get rid of all the other organelles and cell wall remnants). However, the mitochondria don't seem to stay alive. We've considered that they may just get too beat up when sorted, but has anyone else tried a similar expt. (sorting organelles) and either failed or (even better) gotten it to work? Thanks, do ----------------------------------------------- Douglas P. Olson Experimental Hematology/AIDS Research Center Massachusetts General Hospital Harvard Medical School 149 13th Street Boston, MA 02129 (617)724-2668 - Phone (617)726-4691 - Fax Dolson4@partners.org - Email
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