> >Hi all > >Has anyone ever found that the EDTA in commercial sheath buffers interferes with >calcium reponses when using BD benchtop cytometers? I always assumed that there would >be so little mixing of the sample and sheath fluid before the interegation point that >I wouldn't make any difference. > >Any insights? > We routinely use Biosure sheath fluid containing EDTA in our EPICS 753 and FACScan. Calcium flux experiments done on both instruments with this sheath fluid using indo-1 or calcium green and fura red worked fine. Justin Fishbaugh University of Iowa Flow Cytometry Facility
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