Ca++ flux sheath buffer

From: Simon Monard (smonard@trudeauinstitute.org)
Date: Mon Nov 13 2000 - 10:44:02 EST

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Hi all

Has anyone ever found that the EDTA in commercial sheath buffers interferes with
calcium reponses when using BD benchtop cytometers? I always assumed that there would
be so little mixing of the sample and sheath fluid before the interegation point that
I wouldn't make any difference.

Any insights?



Simon Monard
FACS Lab Manager
Trudeau Institute
Saranac Lake
NY12983

Ph 518 891 3080 X352

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