Hi, I've been phenotyping cells from the popliteal lymph node in NIH mice, and in all animals (also untreated mice), I see two distinct populations in the FS/SS histogram. The two populations differ in FS (*2), but does not seem to be dead cells or dublets (controlled by microscopy). Both cellpopulations do display CD3+ cells, indicating that they are lymphocytes. Have anyone seen this while analysing lymph node cells, and, if so, did you find the characteristics of these popluations? I woud appreciate to hear about all similar experiences! Thank you! Best regards, Unni C. Nygaard Unni Cecilie Nygaard National institute of public health PB. 4404 Torshov N-0403 Oslo T: +47 2204 2662 unni.cecilie.nygaard@folkehelsa.no
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