Jeff Barry wrote: > >> A colleague is trying to look for apoptosis using the sub G1, Propidium > Iodide, method however, his cells stubbornly refuses to form single cells > and continue to aggregate causing our FacsCalibur to block every time we put > the samples on. Can anyone relieve our misery by suggesting any sample > preparation tips that will prevent cell clumping. > A variation on this assay utilizes hypotonic lysis to isolate individual nuclei instead of analyzing whole cells. It is described in: J Immunol Meth, 139: 271-279, 1991. This should eliminate cell/cell binding. Good luck, Tom ***************************************************************************** Thomas W. Mc Closkey, Ph. D. Director of Flow Cytometry, North Shore University Hospital Assistant Professor of Pediatrics, New York University School of Medicine Boas Marks Biomedical Research Center, 350 Community Drive Manhasset, Long Island, New York 11030 ph: 516-562-4844 [office], 516-562-1135/4641 [lab] fax: 516-562-2866 *****************************************************************************
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