Macie, I have never had a problem with using the same isotype, only when the target epitopes are too close and there is either steric hindrance or energy transfer should you have a problem with directly conjugated antibodies. Randy Fischer NIH/NIAMS Building 9 Bethesda, MD 20892 fischer1@mail.nih.gov (301) 594-3537 > ---------- > From: Maciej Simm > Sent: Monday, October 16, 2000 4:15 PM > To: Cytometry Mailing List > Subject: Isotype question, refined > > > greetings, > > My question was unclear to some so I'm rewording it, sorry for the > confusion: > > the antibodies are of the same isotype, IgG1, but different > specificities and fluorochromes, ex. antiCD3-FITC-MouseIgG1 with > antiCD4-PE-MouseIgG1 in the same tube. Or would it be better to use > IgG1 and IgG2a, the way BD mixes their simulset reagents? > > > > __________________________________________________ > Do You Yahoo!? > Yahoo! Messenger - Talk while you surf! It's FREE. > http://im.yahoo.com/ > >
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