Stojan Dimitrov wrote: > > > > 1. Is it possible to activate CD4 and CD8 cells in > whole blood with only specific peptide/protein w/o MHC > presenting cells? To my knowledge, you need APC for this reaction to occur. There may be some level of T-T Ag presentation but it is small to nonexistent. The B cells and monos do a much better job of Ag presentation. By providing additional costimulation [throgh CD28 and CD49d] you will enhance the level of response. > Is there any differences b/w CD4 and CD8? Yes, it is possible to use Class I restricted peptides which will only elicit a CD8 response. There are also Cl II resctricted antigens. Just as there are cl. I & II restricted tetramers. Other [generally larger proteins] antigens will elicit some level of response through both pathways [ie you will see CD4 and CD8 responsers] > 2. If it is possible Where we may purchase this > antigens? > The NIH has a selection of specific antigens available. Good luck, Tom ***************************************************************************** Thomas W. Mc Closkey, Ph. D. Director of Flow Cytometry, North Shore University Hospital Assistant Professor of Pediatrics, New York University School of Medicine Boas Marks Biomedical Research Center, 350 Community Drive Manhasset, Long Island, New York 11030 ph: 516-562-4844 [office], 516-562-1135/4641 [lab] fax: 516-562-2866 *****************************************************************************
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