Dear flowers, Sorry for my English ,but I'll try to explain what we need it. In Bulgaria we carry on antiretroviral therapy(HAART) and in our laboratory we monitoring some basic populations and many other subpopulations. But now we want to measure specific CD4 and CD8 cells. We'll purchase a BD kit for this pupose: Fastimmune Cytokine Sistem. Now there is three way to make this: ELISPOT, tetramer complexes and this intracellular cytokine assay. In other to measure specific CD4 and CD8 cells we need an specific peptides/proteins. In this kit there is no specific antigens(peptides/proteins) for this purpose. I have the following questions: I would be vary grateful, if you gave me theoretical articles not only practical suggestions. 1. Is it possible to activate CD4 and CD8 cells in whole blood with only specific peptide/protein w/o MHC presenting cells? What about theoretical explanation? Is there any differences b/w CD4 and CD8? 2. If it is possible Where we may purchase this antigens? Thank you in advance. Stoyan Dimitrov Central Laboratory of Immunology National Center of Infectious and Parasitic Deseases 1504 Sofia, 26, Yanko Sakazov Blvd, Bulgaria __________________________________________________ Do You Yahoo!? Get Yahoo! Mail - Free email you can access from anywhere! http://mail.yahoo.com/
This archive was generated by hypermail 2b29 : Sat Mar 10 2001 - 19:31:34 EST