Re: Cell Volume Revisited

From: David Galbraith (dgalbrai@ag.arizona.edu)
Date: Sun Oct 15 2000 - 08:24:52 EST


We described a circuit that takes the time-of-flight signal and squares or
cubes it (Galbraith, D. W. (1990). Isolation and flow cytometric
characterization of plant protoplasts. In: Methods in Flow Cytometry (Z.
Darzynkiewicz, H.Crissman, eds.), Methods in Cell Biology 33:527-547).
Voila! -- real-time measurement of cell surface area or volume (assuming
cell is spherical, of course, but many are when treated with proteases to
release them from surfaces).

David Galbraith



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