>Does anyone have any information on how to set up the Vantage for DNA >cell cycle using Hoechst. I know that BD has a QC kit using PI >staining. Do I set up the instruement using this kit and then switch to >the FL5 channel for Hoechst? I haven't been able to find out any >information on setting up the Vantage for Cell cycle using Hoechst. >Thank you >Gayle Gayle, We use Hoechst 33342 beads from Flow Cytometry Standards Corp. or Rainbow Control Particles RCP-30-5 from Spherotech to align and set up the Vantage. Recently, we have looked at using the DNA QC Kit from BD for quality assurance. The preliminary data suggest a final Hoechst 33342 concentration of between 2 and 10 µg/ml works well with the CTNs from the kit. Good luck, Eric /\/\/\_ Eric Van Buren, aa9080@wayne.edu \ \ \ Karmanos Cancer Institute and Immunology & Microbiology \_^_/ Wayne State University, Detroit, Michigan, USA
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