Look up: Papa S and Valentini M. Functional NK assays using flow cytometry. Methods in Cell Biology, 42: 193-206, 1994. This procedure uses PI to detect dying cells. Wierda WG, Mehr DS and Kim YB. Comparison of fluorochrome-labeled and 51-Cr-labeled targets for natural killer cytotoxicity assay. J Immunol. Methods, 122: 15-24, 1989. This procedure uses bis (carboxyethyl) carboxyfluoroscein to label targets and determines the loss of labeled cells. I modified the second procedure using fluoroscein diacetate succinimide ester (from Molecular Probes) as the label, since it covalently attaches to proteins and does not leak out of the target cells. This was more sensitive than 51-Cr release especially with NK resistent targets. Tony Bakke, PhD Oregon Health Sciences University >>> Bob Donahoe <rdonaho@emory.edu> 09/15 12:44 PM >>> I'm hoping that someone can direct me to a viable protocol for measuring NK-cell activity by flowcytometry. And, how does such measure-up to the standard chromium release assay? Thanks Robert Donahoe Laboratory of Psychoimmunology Emory University
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