The FS/SS primary gating technic tended to include non-blast cells (mainly lymphocytes) within it. That was one of the reasons the 20% threshold was applied to conclude a blast "positive". However, the CD45/SS gating excludes the lymphocytes. There may be a small number of non-leukemic blasts in the gate, but the rest are all leukemic blasts. For those using CD45/SS gate, what is your threshold for positivity? Many thanks... Bakul I. Dalal, MD FRCPC FCAP FASCP Associate Director, Hematology Laboratory Director, Flow Cytometry Laboratory Vancouver, General Hospital Clinical Professor, Faculty of Medicine University of British Columbia 910, West 10th Avenue Vancouver, BC, Canada, V5Z 4M3 604 875 4496/4633(f)/877 5949(pgr)
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