Hi Flow Jockies, My question is about left-shifting of samples. I have a problem where unstained cells (my voltages and compensations are set from this specimen) and isotype controls run nicely but I get an extreme left-shift in one channel with experimental cells (today it was in FL-1 with human CD61-FITC). I can bring the pixels off the axis by raising the voltage in FL1, but then the read is off when I go back to cells only and isotypes (they are right shifted). The SSC and FSC did not change and I got nice signal in FL2 with CD38-PE. This implies "anti-fluorescence" to me, for lack of a better word, in FL1. I don't know what to make of it. Any comments would be most welcomed. Best wishes, Willy Lensch
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