> ---------- > From: hkleewk@netvigator.com > Sent: Saturday, 19 August 2000 11:58 > To: Cytometry Mailing List > Subject: CD45 Negative/Dim leukaemia case > > On 19/8/00 > WK LEE wrote: > > Dear Flowers, > I've come across a leukaemia case of CD45 dim or maybe negative > expression. > Have any dear friends got experience on this kind of cases? > We've done the 3-color immunophenotyping, using SS/CD45-PC5 as the gating > stretegy. The gated population on bone marrow specimen has positive > expression of CD19, CD10, HLA-DR, CD13, CD34; negative expression of CD14, > CD3, CD5, CD7 and sIg. Weak expression of CD13 was also observed. We're > waiting for the t-dT result by immunohistochemical detection. Would you > consider these findings are case of biphenotyping leukaemia or ALL with > abberrant expression of myeloid marker?? > > WK LEE > > -------------------------------------------------------------------------- > ------------------ > > I work in a paediatric hospital and see quite a lot of the Common/Pre-B > ALL phenotype you descibe. > I find about 5-10% are CD45 dim-negative. The weak CD13 staining I find to > be the most common aberrant marker and report it as such. Weak cyto IgMu > will destinguish the Pre-B ALL although I dont bother with this i/c stain. > I include a few more B-cell markers in the panel- CD22, CD9 (early) and > CD20, CD21 (late) to help stage the ALL. > Biphenotypic leukemias must have several lineage markers eg, > myeloperoxidase + CD13 and CD10 + CD19 + CD22 on the same cells. > > Regards, > Greg Hodge. >
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