Mary Stewart wrote- >Does anyone have experience using LDS-751 for cell cycle analysis? I'm >looking for a cell permeant dye that will detect DNA in live GFP positive >or negative cells. We have a BD FACScan with a 488 argon laser, so I'm >limited to what can be used with this laser. Thanks for any help. LDS751 is not DNA specific. What you want is a new dye, DRAQ5, described by Paul Smith <smithpj2@cf.ac.uk> et al in the August 1, 2000 issue of Cytometry (40:280-291); this is the first dye of which I am aware that gives reasonable DNA histograms from live cells with 488 nm excitation. -Howard
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