You might want to try DRAQU5 (see Paul J Smith in Cytometry 40:280-291 (2000). Whilst better excited with the HENE, it also seems to to work with 488nm excitation and w/o fixing the cells. Good luck Gerhard -----Original Message----- From: Hirofumi Akari [SMTP:hakari@niaid.nih.gov] Sent: Wednesday, August 09, 2000 9:59 PM To: Cytometry Mailing List Subject: Re: GFP/DNA Dear Bill, How about 7-AAD? This fluorescence is detected by FL3, which requires least level of conpensation with GFP. I hope this will help you. Sincerely, At 2:57 PM -0400 8/8/00, William Ross wrote: > what's a good dye to use for simultaneous DNA and GFP detection when your > UV laser has taken an extended vacation? cardiac smooth muscle cells. > > Much thanks. > > Bill Ross ***************************************************** Hirofumi Akari, D.V.M. & Ph. D. Viral Biochemistry Section, Laboratory of Molecular Microbiology National Institute of Allergy and Infectious Diseases National Institutes of Health, 4/312, Center Drive MSC, Bethesda, MD 20892 phone: 301-496-3132, fax: 301-402-0226 *****************************************************
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