what's a good dye to use for simultaneous DNA and GFP detection when your UV laser has taken an extended vacation? cardiac smooth muscle cells. Much thanks. Bill Ross William (Bill) Ross Director - FACS Core Facility MR-4, Box G005 University of Virginia School of Medicine Charlottesville, VA 22908 office (804) 982-1586 fax (804) 924-1221 e-mail:wgr8w@virginia.edu REPLY -----Original Message----- From: Gary Warnes [mailto:g.warnes@csc.mrc.ac.uk] Sent: Thursday, August 10, 2000 8:41 AM To: cyto-inbox Subject: >Hi Bill, >I have used the method published by Chu in Cytometry 36, 333-339 (1999), >bascically ice-cold 1% PFA incubated with cells on ice for 1h then the 70% >ethanol step etc and finally PI. I have found that for some cell types you >do get leakage, COS cells were fine. > > >Gary Warnes, >Head of Flow Cytometry at Fluorescent Imaging >MRC, Hammersmith Hospital, >London, UK
This archive was generated by hypermail 2b29 : Sat Mar 10 2001 - 19:31:29 EST