Hi, Folks I work with have successfully detached murine and rat endothelial cells with collagenase (since these cells were grown on a collagen matrix). These cells maintained their "stainability" for CD31. Am interested in but have not yet tried the reagents available at Phoenix Flow Systems: http://www.phnxf Cheers, Sam Sam Witherspoon sw11527@glaxowellcome.com Dept. of Receptor Biochemistry Tel. 919-483-3078 Glaxo Wellcome R&D Page 919-857-7768 5 Moore Dr. Fax 919-483-0585 RTP, NC 27709 > -----Original Message----- > From: Thanos P Kakoulidis [SMTP:kakoulidis@louisville.edu] > Sent: Monday, August 07, 2000 7:10 PM > To: Cytometry Mailing List > Subject: detaching EC > > Hi Flowers, > > does anybody know of another method except trypsinization, to detach > endothelial cells from the flask? (that is necessary in order to create a > single cell suspension and run the cells in the flow cytometer) > > thanks, > > thanos > > Thanos Kakoulidis, MD > Ph.D. Graduate student > Department of Microbiology and Immunology > Institute for Cellular Therapeutics > University of Louisville
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