Dear flowers, well, I ran some more samples and here is what I found: the monocytes sorted with magnetic anti-CD14 but NOT stained are a tad brighter (2 clicks on the log scale) vs. the unstained lymphocytes (sorry, no unstained monos in this batch) in both FL1 and FL2 (I didn't study any other channels) anti-isotype antibody for the MACS anti-CD14 antibody pics up signal in the 2nd decade (same instrument settings as isotype control on monos) this proves magnetic CD14 antibodies stay on the sorted cells and so polyclonal secondary antibody should not be used when magnetic separation is used (duh.) I'll keep the group posted if anything else comes up along this topic. As far as recommended vendors of antibodies I asked for in the original post, I'll compile a post next week b/c I'm still getting new emails. regards, Maciej __________________________________________________ Do You Yahoo!? Kick off your party with Yahoo! Invites. http://invites.yahoo.com/
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