anti-biotin background

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RE: anti-biotin background (was: Amplification techniques (WAS Question
regarding CD40 surface expression level by FACS))

Can anybody explain why anti-biotin has a lower background staining than
avidin compounds?
> ----------
> From:		Joseph Mattapallil
> Sent:		Wednesday, August 2, 2000 6:34 PM
> To:	Cytometry Mailing List
> Subject:	Re: Amplification techniques (WAS Question regarding CD40
> surface expression level by FACS)
>
>
> Regina hi
>
> One of the things that might work would be to use a biotinylated
> primary antibody and then come back with anti-biotin FITC. The
> background is in fact quite less as compared to streptavidn or
> avidin. Vector use to make an anti-biotin FITC. I am not sure if
> anybody makes anti-Biotin PE. If any body knows I would be interested
> in finding out. Hope this helps.
>
> Joseph Mattapallil
> Asst. Research Immunologist
> School of Medicine
> University of California Davis
> Davis, CA 95616
>
>

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