Re: viability issue on the Moflo cell sorter

From: janet dow (jldow@unity.ncsu.edu)
Date: Wed Aug 02 2000 - 08:17:37 EST


At 4:40 PM -0400 7/31/00, Nathalie Tessier wrote:
>Greetings to all flowers
>
>
>We are going to buy a high speed cell sorter and are really considering
>the Moflo. Somebody raised the issue of viability when sorting fragile
>cells with the Moflo. I would like to know if any Moflo users
>encountered that problem. Any information would be very usefull to us.
>
>thanks in advance
>
>--
>Nathalie Tessier, M.Sc.
>Head of Flow cytometry Service
>Institut de recherches cliniques de Montréal
>110 west, Pine Avenue
>Montreal, Quebec, Canada
>H2W 1R7
>tel: 514-987-5608
>fax:514-987-5736

We sort cat lymphocytes from both lymph nodes and blood through a MoFlo and
have had excellent viability and recovery-on the order of 85-90% viable.

hope this helps

Janet Dow

Janet Dow
Research Technician and Manager
Flow Cytometry Facility
North Carolina State College of Veterinary Medicine
Room C-314
Raleigh, NC 27606
(919)513-6364



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