On Wednesday, 26 July 2000, Shannon C. Ritchie (sritchi@emory.edu) asked: >>I have heard of using trypan blue to reduce background. Does anyone know the concentration and protocol? Try the following reference: "Reducing Cellular Autofluorescence in Flow Cytometry: An In Situ Method" Mosiman et.al., Cytometry (Clinical Communications in Cytometry) 30:151-156 (1997). There was also an article in Cytometry by Amersfoort and Strijp (Cytometry 17:294) examining different brands of trypan blue and quenching efficiency. Steven Merlin Associate Director Hospital for Special Surgery Flow Cytometry Core Facility Caspary Research Bldg., Lab 312 541 East 71st Street New York, N.Y. 10021 Tel: 212-606-1925 Pager: 212-606-1644 / 916# E-mail: merlins@hss.edu <mailto:merlins@hss.edu> Website: http://hss.hss.edu <http://hss.hss.edu>
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