On Thu, 20 Jul 2000, Scott Tighe wrote: > 1) Has anyone used the reagent called Phi Phi Lux for apoptosis > detection? If so, where can I get it? Hi Scott, I have used a couple of the PhiPhiLux variants (the 'green' and 'orange' versions) with some success - you may have to play with the conditions (substrate concentration etc) and it is worth looking at different time points after apoptotic induction. PhiPhiLux comes from OncoImmunin and there is a website at: http://www.PhiPhiLux.com > 2) Also I learned (at the flow coarse in maine)that someone has > recently come out with a 488nm exc viable DNA stain similar to 33342. I > think Jim Jacobberger had mentioned it to me. Does anybody have any > idea if it is for real or available? I assume you are referring to DRAQ5 from Paul Smiths group in Cardiff? Check out: Smith et al (1999) JIM, 229, 131-139 and Wiltshire et al Cytometry (2000), 39, 217-223. Derek ************************************************************************ Derek Davies Voice: (44) 020 7269 3394 FACS Laboratory, FAX: (44) 020 7269 3100 Imperial Cancer Research Fund, e_mail: derek.davies@icrf.icnet.uk London, UK mobile: 07790 604112 Web Page: http://www.icnet.uk/axp/facs/davies/index.html In tenebris lux *************************************************************************
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