Dear flowers! Has anyone tried to freeze murine spleen cells before doing intracellular cytokine staining on these thawed cells? And if, which freeze and thaw procedure have you been using? Is there some problems we need to be aware off? A last question, which perm & fix method is to be preferred when working with murine cells doing intracellular cytokine staining? Thanks in advance! Anita Anita Haugan Department of Vaccinology, VAFU National Institute of Public Health, Norway
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