Greetings, I have a client who would like to sort potato protoplasts. We are wondering if anyone out there does this and if so if anyone has any input into whether Hoechts or DAPI would be a better choice. Do both of these dyes work without affecting the viability of the cells. They definately want live cells when we are done. What about 7-AAD. We currently run a lot of 7-AAD in our lab looking at apoptosis. I know it is used as a DNA dye. Does it provide ploidy peaks? If so does anyone have any input as to whether this dye would be a good choice for this undertaking. One other question while i'm at it. For the DAPI and Hoechts, what filters would you recomend using to measure emission? In the literature they had, there were conflicting reports of wavelengths. Any and all input will be appreciated and put to good use. Thanks in advance for all help and responses. Joan K Flow Cytometry Lab Supervisor VMRCVM (540) 231-4115 FAX 540-231-7367 jkalnits@vt.edu "It is better to serve than to receive." B. Borg
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