more on CD45: the cells in our tubes are NOT basophils, simple math - if you take 2 tubes: cd3/cd19 cd3/16+56 you will know how many B,T and NK cells you have in your light scatter gate (or ssc/45) B+T+NK=100% lymphs (more less) thus the -/- cells in tube 1 should be approx. the number of NK cells, and the -/- cells in tube 2 should be approx. the number of B cells. knowing the CD19 and 16/56+ populations I can check -/- from tube 2 vs. my CD19+ cells and vice versa with CD16/56. If the numbers match, can I say that all my lymphocytes are accounted for ? UNLESS basophils express CD3?? Therefore, in a tube that has 40% dim CD3's but has B,T and NK cells add up to around a 100, there cannot be 40% basophils. Something else is causing the dimness in CD45. Does this make sense to anyone? PS I will update my site soon - been too busy with med school applications. Thanks again to all of you who took time to correspond. Maciej __________________________________________________ Do You Yahoo!? Send instant messages & get email alerts with Yahoo! Messenger. http://im.yahoo.com/ -- End --
This archive was generated by hypermail 2b29 : Sat Mar 10 2001 - 19:31:25 EST