second laser for a MoFlo

From: Isabell Schmitt (schis@pei.de)
Date: Tue Jul 04 2000 - 09:06:33 EST


Hello Fellow Flowers!

I have an urgent question regarding the laser equipment for a MoFlo.
I had posted a question a couple of weeks ago concerning a second laser for simultaneous
excitation in the visible and UV and was very thankful for all the answers . Now the
company offered us the combination of one Innova 70C - 5 watt laser and as second
laser an Innova 70C - 2 watt laser. As I had gotten a lot of comments from people
having two 5 watt lasers I wonder weather the power of 2 watt is enough for a second
laser or weather it would be better to have two 5 watt lasers?
(we want to sort for cells stained with FITC, PE, PE-Cy5, APC (red diode is already
included), DAPI besides others, but also cells expressing (alone or in combination)
EGFP, EYFP, EBFP, ECFP and/or  DsRed.

Thanks a lot in advance!!!

Isabel

Isabel Schmitt
Dept. Medical Biotechnology
Paul Ehrlich Institute
Paul Ehrlich Str. 51-59
D-63225 Langen
e-mail: schis@pei.de



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