Dear Flowers, I need some help with mouse CD3 antibodies. I have an investigator who was doing CD4/CD8 on mouse spleen and thymus very nicely until she decided she wanted to first gate through CD3. We have tried two CD3 antibodies with mixed results. The 500-A2 clone from Caltag didn't stain most of the CD8 positive cells. The 145-2C11 from Pharmingen stains pretty well but still includes a lot of non CD4/CD8 cells in the gate. Where to gate is not completely clearcut. I am gating against forward scatter. Since I don't have a lot of experience with Tcell staining, I wasn't sure what I should expect, but thought it should do better than that. I am hoping those of you with mouse experience will share with me your expertise. Also, what is a good resource that discusses these markers in mouse or human? Thanks, Candace Enockson Medical University of South Carolina
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