Hi Al, These compounds, if my memory serves me correctly, are best suited to time based applications (due to their long fluorescence lifetimes) and can be used where one wishes to obtain a signal above background, one would let the backgorund "quench" and then read the remaining signal : can be kind of difficult to do in flow! The guy was probably right about the narrow emission, as this is one of the characteristics of these compounds. Your might like to have a little look here http://violet.berkeley.edu/~minli/postdoc.html Sorry, I have no other gems on this topic Geoff >Flowers-- > >I had a researcher come into the lab with a Eu-chelate as the >fluorochrome. He came without literature and said it had an excitation >peak around 345nm (I used the relatively weak 351nm line from an >Enterprise) and a very narrow emission at 615nm (I used a 610/20 to >collect). I was unable to see any kind of signal although his controls >using a FITC-conjugate worked fine. I was a little suspicious when he >said it was "quantumly inefficient" and he had never seen it tried on a >flow cytometer (I'm betting it might need a little more time in the >beam). Do any of the gurus out there have any wisdom to share. > >Al > >asaluk@scripps.edu ===================================================== Geoffrey Osborne Manager, Multi User Flow Cytometry Facility Biomedical Research Centre, 2222 Health Sciences Mall, University of British Columbia Vancouver B.C V6T 1Z3 CANADA Email: geoff@brc.ubc.ca Phone: 604 822 7838 Surfing the Web?: Try http://jcsmr.anu.edu.au/facshome.html or http://www.cytometry.brc.ubc.ca =====================================================
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