Due to the dye transporter systems the only working system for me has been carboxy-fluorescein-diacetate-succinimidylester. However in the absence of dual excitation ratio (as described in Howard Shapiro's book) your measurements are severely limited due to variations in the dye loading and cell volume => width of the distribution. Thus you only get a rough estimate. Unfortunately you can also not work in the exponential growth phase as that won't allow you dye loading in the first place. All a bit limited, the whole method unless you have a non-pumping strain.. good luck Gerhard -----Original Message----- From: Elaine Kunze [SMTP:mek4@psu.edu] Sent: Wednesday, June 21, 2000 9:37 PM To: Cytometry Mailing List Subject: bacterial pH Has anyone measured pH in E. coli using Lysosensor Green? If not, what is a good alternative for measuring pH? **************************************************************************** Elaine Kunze Flow Cytometry.....Image Analysis... Life Sciences Consortium 8B Althouse Laboratory (814-863-2762) Penn State University University Park, PA 16802
This archive was generated by hypermail 2b29 : Sat Mar 10 2001 - 19:31:24 EST