Re: proliferative responses

From: Betty Anne McBey (bmcbey@ovc.uoguelph.ca)
Date: Wed Jun 21 2000 - 07:43:41 EST


	I've been using Alamar blue in my blastogenesis assays which you can
read in either an ELISA reader or even better in a fluorimeter.  It's
available from Trek Diagnostics, phone # 1-800-871-8909.  They can give
you information on the dye or if you want information on how I use it,
just email me.
	Betty-Anne

Jose Benito wrote:
>
> Hi eveyone. Sorry, this is not a flow question but does anyone know of a
> fluorimetric-based technique to measure lymphoproliferative responses in
> response to mitogens and antigens?. Molecular Probes has a kit called
> "Cyquant" that measures DNA content using a DNA-binding green dye. Does
> anyone have some experience using this kit?
> Thanks
> Jose Miguel Benito
> Hospital Carlos III
> Madrid
> Spain
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