Hi,
I think B-D sorters use .18mm internal diameter 2mm external diameter
silicone or similar tubing, the nearest I've ever got to this is
.254mm id silicone tubing from a company called Altec in the UK
(http://www.altecweb.com/asp/B_listcategory.asp?categno=1500&rangeno=E05&rangetitle=AlteSil%99+Silicone+Rubber+Tubing).
It's MUCH cheaper than the B-D stuff (approx two pounds per metre
rather than tens of pounds per piece from B-D), but you'll find that
you need a lower sample differential pressure to get an acceptable
cell flow rate. You might need to pre-stretch one end of the tubing
to get it to slide onto the nozzle holder easily, if so you can
insert the tip of a pair of fine forceps as far as you can and leave
over-night to stretch.
It's important that you choose a soft plastic like silicone or
perhaps Tygon for the sorter sample tubing, because the pinch valve
isn't strong enough to clamp off the flow for polystyrene, polythene,
pvc or ptfe (teflon) when you put the machine into standby (which
means that the sample can be diluted very quickly by the sheath
back-flow).
I hope this helps,
Ray
At 1:05 pm -0700 6/6/00, P S Manogaran wrote:
>Dear flow users,
>
>We are running short of BD FACS sample tubing. Ordering it through BD
>takes some time .we are thinking of ordering it through some other
>supplier because it will be quicker and cheaper. I will appreciate, if
>someone provide us the information about inner and outer diameter
>of the sample tubing and name of suppliers who could supply us the
>materials.
>
>Thanks in advance
>
>P.S.Manogaran.
>
>Flow cytometry facility
>Centre for Cellular and Molecular Biology
>Hyderabad-500007
>INDIA
--
Ray Hicks
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