The supplier is: Aurora Biosciences (US Phone: (858) 404-6600 http://www.aurorabio.com/ It works in flow (we do it), but I don't know if it is permeable into bacteria. CCF2 is a FRET dye so you collect both blue (cleaved) and green (non-cleaved) fluorescence channels to measure activity. Phil Marder >From: "isabelle.hautefort" <isabelle.hautefort@bbsrc.ac.uk> >To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu> >Subject: B-lactamase assay in individual bacteria? >Date: Tue, 6 Jun 2000 12:51:37 +0100 > > >dear all, >I would like to detect B-lactamse activity in individual bacterial cells >so >that I can control the presence of a (AmpR) plasmid and check if its copy >number is rather similar in every cell. I know that there is a fluorescent >substrate for b-lactamase (CCF2 like). Does anyone of you know whether it >works >in a flow cytometer (which channel?) and which supplier sales it? (not >Molecular Probes, at least not yet). >Thanks for your help. >Regards >Isabelle > >********************************************************************* >Dr. Isabelle Hautefort >Molecular Microbiology Section >Institute of Food Research >Research Park- Colney >Norwich NR4 7UA- UK > >Tel +44(0)1603 255 391 >Fax +44(0)1603 458 414 >E-mail: isabelle.hautefort@bbsrc.ac.uk >********************************************************************* > ________________________________________________________________________ Get Your Private, Free E-mail from MSN Hotmail at http://www.hotmail.com
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