dear all, I would like to detect B-lactamse activity in individual bacterial cells so that I can control the presence of a (AmpR) plasmid and check if its copy number is rather similar in every cell. I know that there is a fluorescent substrate for b-lactamase (CCF2 like). Does anyone of you know whether it works in a flow cytometer (which channel?) and which supplier sales it? (not Molecular Probes, at least not yet). Thanks for your help. Regards Isabelle ********************************************************************* Dr. Isabelle Hautefort Molecular Microbiology Section Institute of Food Research Research Park- Colney Norwich NR4 7UA- UK Tel +44(0)1603 255 391 Fax +44(0)1603 458 414 E-mail: isabelle.hautefort@bbsrc.ac.uk *********************************************************************
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