Hallo Helen, we have fighted with a similiar problem-the solution for my knowledge is the possibility, that damaged or dead cells take up in an unspecific manner Tricolor. Levelt, C.N., Eichmann, K.: Streptavidin-Tricolor is a reliable marker for nonviable cells subjected to permeabilization or fixation. Cytometry 15 (1994) 84-86 Helen Horton schrieb: > Dear flowers- > > I have been using tricolor and cychrome- labelled antibodies for > staining mouse splenocytes and have been getting a lot of background > staining (i.e my isotype controls labelled with these fluorescent > markers are sticking). I have tried using Fc block (Pharmingen) from > 2-8ug/sample but this does not appear to be helping much. Can anyone > help? > > Thanks in advance, > > Helen > Helen Horton PhD > Wisconsin Regional Primate Research Center > 1220 Capitol Court > Madison > WI 53715 > Tel: (608) 265 3381 > Fax: (608) 263 4031 > hhorton@primate.wisc.edu
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