Dear flowers- I have been using tricolor and cychrome- labelled antibodies for staining mouse splenocytes and have been getting a lot of background staining (i.e my isotype controls labelled with these fluorescent markers are sticking). I have tried using Fc block (Pharmingen) from 2-8ug/sample but this does not appear to be helping much. Can anyone help? Thanks in advance, Helen Helen Horton PhD Wisconsin Regional Primate Research Center 1220 Capitol Court Madison WI 53715 Tel: (608) 265 3381 Fax: (608) 263 4031 hhorton@primate.wisc.edu
This archive was generated by hypermail 2b29 : Sat Mar 10 2001 - 19:31:18 EST