Dear All We are attempting to set up 4 colour assays on our FACS Vantage (NOT SE upgraded) using APC on a HeNe laser delivering about 30 mW. Abs are from BD. 1) Should we expect to see the APC signal in the FL3 channel (optimised for PECY5) of our 488 nm line? ie. Is APC excited at all by the 488 nm laser? 2) Is anybody using BD's CD11c-APC mAb on a similar system, if so how does the signal compare with PE. Any help or hints on setting up 4 colour on a Vantage with 488 and 635 nm laser lines would be most appreciated. Len Brown
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