I guess FITC would be a good candidate as it could be quenched extracellular by
a number of methods such as low pH or EB.
You might even be able to use the 'snip'method as used in magnetic sorting
Regards
Gerhard
-----Original Message-----
From: zip@biotransplant.com [SMTP:zip@biotransplant.com]
Sent: Thursday, May 04, 2000 6:50 PM
To: Cytometry Mailing List
Subject: Reagents for measurement of antibody internalisation...
Greetings Floweurs (getting ready for ISAC!),
I would appreciate any suggestions of potentially suitable
fluorescent reagents, which could be coupled directly to specific
antibodies & then used to determine internalisation of those
antibodies by B-cells, eg. by appearance or disappearance of
flourescence or by shift in the fluorescence spectrum & then measured
by flow cytometry.
Many thanks,
"Zip".
>>>--->
==================================================================
Huw S. ("Zip") Kruger Gray, Ph.D. | BioTransplant Inc.,
Scientist: Flow Cytometry. | Building 75,
----------------------------------------| Third Avenue,
'Phone: (617)-241-5200, extn: 254/256. | Navy Yard,
Fax: " " -8780. | Charlestown,
E-mail: Zip@BioTransplant.com | MA, 02129, U.S.A.
==================================================================
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